How to sterilize vials

Page 1 of 3 123 LastLast
Results 1 to 10 of 29
  1. #1
    Board Rep
    BOARD REP
    Liquidex's Avatar


    Join Date
    Sep 2012
    Location
    In a 30ml plastic bottle :)
    Posts
    1,027
    Rep Points
    -21587565

    Post How to sterilize vials






    Oven
    Dry heat is less effective than steam for sanitizing and sterilizing, but many brewers use it. The best place to do dry heat sterilization is in your oven. To sterilize an item, refer to the following table for temperatures and times required.

    Table 3 - Dry Heat Sterilization

    Temperature Duration

    338°F (170°C) 60 minutes

    320°F (160°C) 120 minutes

    302°F (150°C) 150 minutes

    284°F (140°C) 180 minutes

    250°F (121°C) 12 hours (Overnight)

    The times indicated begin when the item has reached the indicated temperature. Although the durations seem long, remember this process kills all microorganisms, not just most as in sanitizing. To be sterilized, items need to be heat-proof at the given temperatures. Glass and metal items are prime candidates for heat sterilization.

    Some homebrewers bake their bottles using this method and thus always have a supply of clean sterile bottles. The opening of the bottle can be covered with a piece of aluminum foil prior to heating to prevent contamination after cooling and during storage. They will remain sterile indefinitely if kept wrapped.

    One note of caution: bottles made of soda lime glass are much more susceptible to thermal shock and breakage than those made of borosilicate glass and should be heated and cooled slowly (e.g. 5 °F per minute). You can assume all beer bottles are made of soda lime glass and that any glassware that says Pyrex or Kimax is made of borosilicate.


    Autoclaves and Pressure Cookers

    Typically when we talk about using steam we are referring to the use of an autoclave or pressure cooker. These devices use steam under pressure to sterilize items. Because steam conducts heat more efficiently, the cycle time for such devices is much shorter than when using dry heat. The typical amount of time it takes to sterilize a piece of equipment in an autoclave or pressure cooker is 20 minutes at 257° F (125 °C) at 20 pounds per square inch (psi).

    BUY STERILE SEALED VIALS
    You can also buy sealed vials and remove the aluminum cap and then use a syringe needle to vent the stopper and fill will a syringe with a 18 or 15g needle Then recap. This is more expensive to do but if you are only doing it at a small scale then this maybe best for you.

    Please Vet brewers add Ideas to this thread to help noobs keep there glass vials sterile.

  2. #2
    Junior Member


    Join Date
    Dec 2012
    Location
    Arkansas
    Posts
    26
    Rep Points
    173678

    Learn something new everyday

  3. #3
    Board Rep
    BOARD REP
    Liquidex's Avatar


    Join Date
    Sep 2012
    Location
    In a 30ml plastic bottle :)
    Posts
    1,027
    Rep Points
    -21587565

    Quote Originally Posted by jonboy View Post
    Learn something new everyday
    You will learn a lot from my posts my friend.

  4. #4
    Member


    Join Date
    Dec 2012
    Posts
    30
    Rep Points
    1844479

    Is it enough to do it in sterilizer designed to sterilize bottles for newborns?

  5. #5
    Board Rep
    BOARD REP
    Liquidex's Avatar


    Join Date
    Sep 2012
    Location
    In a 30ml plastic bottle :)
    Posts
    1,027
    Rep Points
    -21587565

    Quote Originally Posted by szatas View Post
    Is it enough to do it in sterilizer designed to sterilize bottles for newborns?
    In my honoest opinion, No. Baby bottle sterilizers kills bacterias that baby's stomach acid cant kill, and leaving anything that may be killed by baby's stomach acid. But sterilizing injectable are TOTALY different story.

  6. #6
    Super Moderator
    SUPER MOD
    GSRacer's Avatar


    Join Date
    Jun 2012
    Location
    Anywhere it's cold and snowy
    Age
    32
    Posts
    4,680
    Rep Points
    2147483647

    Quote Originally Posted by Liquidex View Post
    In my honoest opinion, No. Baby bottle sterilizers kills bacterias that baby's stomach acid cant kill, and leaving anything that may be killed by baby's stomach acid. But sterilizing injectable are TOTALY different story.

    ^^this.
    I only use compounds verified for proper composition and concentration through spectroscopy conducted in a sterile laboratory.

  7. #7
    Member


    Join Date
    Nov 2012
    Posts
    41
    Rep Points
    491013

    how can you sterilize rubber stoppers also?

  8. #8
    Board Rep
    BOARD REP
    Liquidex's Avatar


    Join Date
    Sep 2012
    Location
    In a 30ml plastic bottle :)
    Posts
    1,027
    Rep Points
    -21587565

    Quote Originally Posted by JJR View Post
    how can you sterilize rubber stoppers also?
    First dip them in 10% bleach, then leanse with 70% rubbing alcohol. Pharmasuitacle companies do it by radio sterilization and dont even have to touch alcohol, but obviously we cant do that unless you have accesse to the nuclear machine.

  9. #9
    Senior Member
    exphys88's Avatar


    Join Date
    Jun 2012
    Posts
    260
    Rep Points
    44192098

    How to sterilize vials

    Quote Originally Posted by Liquidex View Post
    First dip them in 10% bleach, then leanse with 70% rubbing alcohol. Pharmasuitacle companies do it by radio sterilization and dont even have to touch alcohol, but obviously we cant do that unless you have accesse to the nuclear machine.
    And they need to dry, it's the process of drying that allows the alcohol to kill the bacteria.
    And 70% alcohol is the most effective, that's why it's the most common.

  10. #10
    Member


    Join Date
    Nov 2012
    Posts
    41
    Rep Points
    491013

    would dipping the rubber into 70% alcohol then covering then until dry be effective? Im just trying to figure out all the steps and how to xactly do it.

Similar Threads

  1. Pinz and vials
    By Vicious 13 in forum Source Checking & Discussion
    Replies: 29
    Last Post: 03-02-2013, 10:09 PM
  2. Reusing vials
    By Deuce in forum Anabolic Steroids
    Replies: 10
    Last Post: 09-17-2012, 03:33 AM

Posting Permissions

  • You may not post new threads
  • You may not post replies
  • You may not post attachments
  • You may not edit your posts
  •  
Site Hosted in Panama


END DISABLED -->